An Adenosine Triphosphate-dependent Deoxyribonuclease from Hemophilus influenzae Rd

An Adenosine Triphosphate-dependent Deoxyribonuclease from Hemophilus influemae

The ATP-dependent DNase of Hemophilus injfuenzae Rd acts as a DNA-dependent ATPase which splits ATP into ADP and inorganic phosphate in the presence of magnesium ions and DNA. The apparent K, for ATP in the ATPase reaction is 0.18 mM. Approximately 40 ATP molecules are utilized for each DNA phosphodiester bond cleaved. All of the common ribose and deoxyribose nucleoside 5’-triphosphates can rep...

Adenosine triphosphate-dependent deoxyribonuclease in Bacillus subtilis.

Mechanism of DNA degradation by the ATP-dependent DNase from Hemophilus influenzae Rd.

The rate of production of acid-soluble material during degradation of duplex DNA by Hemophilus influenzae ATP-dependent DNAse (Hind exonuclease V) has been shown to be directly dependent upon the Mg2+ concentration in the reaction mixture. At high concentrations of Mg2+ (5 to 20 mM), DNA degradation to acid-soluble products is rapid and the rate of ATP hydrolysis is slightly depressed. At low c...

The Isolation and Characterization from Escherichia coli of an Adenosine Triphosphate-dependent Deoxyribonuclease Directed by ret

Extracts of wild type Escherichia cofi contain a DNase which catalyzes the release of acid-soluble fragments from native DNA; this activity cannot be detected in ret B and C mutants. The properties of this enzyme have been studied with a fraction purified ZOOto 600-fold. Only in the presence of ATP does the purified enzyme act on native DNA; with denatured DNA this ATP requirement is not absolu...

Adenosine Triphosphate-dependent Taurocholate

Transport systems involved in uptake and biliary secretion of bile salts have been extensively studied in rat liver; however, little is known about these systems in the human liver. In this study, we investigated taurocholate (TC) transport in canalicular and basolateral plasma membrane vesicles isolated from 15 human livers (donor age 6-64 yr). ATP stimulated the uptake ofTC into both canalicu...